1/62. A Japanese case of dengue fever with lymphocytic vasculitis: diagnosis by polymerase chain reaction.A 37-year-old Japanese male was admitted to Nagasaki University Hospital with abrupt onset of biphasic fever, general malaise and myalgia 9 days after coming back to japan from Manila. He developed a rubella like erythematous rash 3 days after admission and purpuric eruption one week after admission. A biopsied specimen from the purpura revealed lymphocytic vasculitis with T cell dominance and without immunoglobulin or complement deposition around the blood vessels. RT-PCR analysis on peripheral blood mononuclear cells using dengue virus specific primers confirmed the diagnosis of type 3 dengue fever. PCR analysis using virus specific primers is a rapid and valuable method for making a correct diagnosis of dengue fever.- - - - - - - - - - ranking = 1keywords = virus (Clic here for more details about this article) |
2/62. The guillain-barre syndrome following dengue fever.A 44 year old female presented with fever, muscle aches, rash and a low platelet count. IgM antibody to dengue virus was positive. Two weeks later she developed a flaccid areflexic quadriparesis. Nerve conduction studies showed a predominantly demyelitinating sensory motor polyneuropathy consistent with guillain-barre syndrome. Despite the relatively common occurrence of dengue fever, an associated polyradiculoneuropathy is distinctly uncommon.- - - - - - - - - - ranking = 0.5keywords = virus (Clic here for more details about this article) |
3/62. Report of a fatal case of dengue infection with hepatitis: demonstration of dengue antigens in hepatocytes and liver apoptosis.A fatal case of dengue (DEN) infection associated with a spleen rupture and with hepatitis is reported here. Microscopic studies showed numerous areas of spleen rupture with hematomas and revealed necrotic foci in liver samples obtained at autopsy. Although hepatitis was reported in several cases of DEN fever, the mechanism of liver injury remains poorly understood. In this case, immunohistochemistry showed that DEN viral antigens were mostly detected in hepatocytes surrounding the necrotic foci. By in situ detection of dna fragmentation, apoptotic hepatocytes were found to be colocated with DEN virus-infected hepatocytes. These findings suggest that hepatocytes are the major sites of DEN virus replication in the liver and that DEN virus induces apoptosis of hepatocytes in vivo.- - - - - - - - - - ranking = 1.5keywords = virus (Clic here for more details about this article) |
4/62. Rapid diagnosis of primary dengue fever by the immunochromatographic test and by electron microscopy--a case report.A 21-year-old woman presented with an acute febrile illness after a two-week holiday in jamaica. Her symptoms started two days after return, with sudden onset of continuous high fever (> 39 degrees C), dizziness and nausea. Three days later she developed a generalized macular rash, which led to the tentative diagnosis "acute dengue fever." Laboratory confirmation was achieved by demonstrating anti-dengue IgM and IgG antibodies in paired sera; in addition, flavivirus particles were directly visualized by electron microscopy.- - - - - - - - - - ranking = 0.5keywords = virus (Clic here for more details about this article) |
5/62. Acute parotitis due to dengue virus.Acute bilateral parotitis is a common clinical feature of various infectious and autoimmune, metabolic, and drug-related conditions. We describe a unique case of bilateral inflammatory enlargement of the parotid glands in an immunocompetent patient with dengue fever. Evidence of dengue virus in the saliva is also provided for the first time.- - - - - - - - - - ranking = 2.5keywords = virus (Clic here for more details about this article) |
6/62. Unusual association of Kikuchi's disease and dengue virus infection evolving into systemic lupus erythematosus.Kikuchi's disease is a histologically alarming self-limiting condition typically affecting the lymph nodes of young females. A 13-year-old girl was presented with fever, skin rash and cervical lymphadenopathy. On examination she was found febrile, mild pallor was present and she had lymphadenopathy. liver was palpable. Cervical lymph node biopsy showed histiocytic necrotising lymphadenitis (Kikuchi's disease). dengue virus serology for IgG blot showed evidence of seroconversion in serial samples. She was treated with antibiotics and fluconazole and cyclosporin A. During hospitalisation she developed retinal vasculitis. She was reviewed after one month and showed rashes of subacute cutaneous lupus erythematosus. This case can be described to be a triggering event by dengue viral infection causing abnormal immune response leading initially to Kikuchi's disease and later on to systemic lupus erythematosus.- - - - - - - - - - ranking = 2.5keywords = virus (Clic here for more details about this article) |
7/62. First isolation of dengue 3 in brazil from an imported case.The authors report the isolation of dengue 3 virus for the first time in brazil. The patient, resident in Limeira-SP, traveled to nicaragua on May 16th, 1998, where he stayed for two months. Starting on August 14 th he had fever, headache, myalgia, arthralgia, retro-orbital pain and diarrhea. He returned to brazil on August 16th and was hospitalized in the next day. The patient had full recovery and was discharged on August 20th. The virus was isolated in C6/36 cell culture inoculated with serum collected on the 6th day after the onset of the symptoms. The serotype 3 was identified by indirect immunofluorescence assays performed with type-specific monoclonal antibodies. This serotype was further confirmed by polymerase chain reaction analysis. The introduction of a new dengue serotype in a susceptible population is a real threat for the occurrence of severe forms of the disease. The isolation and identification of dengue virus are important in order to monitoring the serotypes circulating in brazil and to take the measures necessary to prevent and control an epidemic.- - - - - - - - - - ranking = 1.5keywords = virus (Clic here for more details about this article) |
8/62. Using buffy coat for reverse transcriptase-polymerase chain reaction in the diagnosis of dengue virus infection: preliminary study.Using reverse transcriptase-polymerase chain reaction (RT-PCR) to detect and type from viremic human serum samples for dengue virus infection is widely used today. However, a few false-negative results were reported due to very low titers of the virus particle in serum samples. As mononuclear cells, macrophages or monocytes are target cells for dengue virus infection, and the replication of virions can be observed in peripheral leukocytes frequently, the amount of virus particle in buffy coat should be higher than those in serum samples. Here, we describe a procedure in which rna extraction from the buffy coat of a patient with a false-negative serum sample yielded specific viral rna amplifiable by RT-PCR, thereby providing an alternative choice for the accurate diagnosis of dengue infection.- - - - - - - - - - ranking = 4keywords = virus (Clic here for more details about this article) |
9/62. Arbovirus studies in two towns in western state of nigeria.Three hundred and fifty-one persons were tested for HI antibody to arbovirus Groups A, B and Ingwavuma viruses in Ilesha and Oshogbo, two towns in western nigeria. Chikungunya accouted for most Group A infections (39%). antibodies to Group B virus were distributed as follows: Dengue 22%, yellow fever 25%, West Nile 28% and Wesselsbron 30%. Few sera 5% were positive to Ingwavuma. No virus was isolated from 188 blood specimens processed for virus isolation.- - - - - - - - - - ranking = 4.5keywords = virus (Clic here for more details about this article) |
10/62. Acute myeloid leukemia precipitated by dengue virus infection in a patient with hemoglobin h disease.We report a unique case of refractory acute myeloid leukemia (AML) precipitated by dengue virus induced marrow aplasia in a Chinese patient with hemoglobin (Hb) H disease. The quick temporal sequence of the three marrow abnormalities: hereditary hemoglobinopathy, reactive viral induced marrow change and malignant neoplastic process is highly unusual. Neither HbH disease nor viral induced marrow aplasia has known associations with AML. We propose that this unique case of AML may be caused by cytokine hyperstimulation in a stressed marrow.- - - - - - - - - - ranking = 2.5keywords = virus (Clic here for more details about this article) |
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