1/4. Uvitex 2B stain for the diagnosis of isospora belli infections in patients with the acquired immunodeficiency syndrome.OBJECTIVE: Fluorescent stains with Uvitex 2B or other fluorochromes are widely used today, especially for the diagnosis of microsporidian infections in human immunodeficiency virus (hiv)-infected patients. Our objective was to ascertain whether the fluorescent stain with Uvitex 2B is also able to detect isospora belli in stool samples and duodenal/bile juice of hiv-infected patients. DESIGN: Case study. SETTING: University hospital of the University of Cologne, germany. patients: Two hiv-infected patients with chronic diarrhea who had intestinal infections with I. belli. methods: Stool was concentrated by a modified water-ether sedimentation method, and duodenal/bile juice was concentrated by centrifugation. Wet-mount preparations were examined by phase-contrast and bright-field microscopy, and smears were stained with a modified acid-fast stain and a fluorescent stain with Uvitex 2B. RESULTS: Using the fluorescent stain with Uvitex 2B, the oocysts of I. belli stained bright white/blue fluorescent and showed a structure similar to that of the oocysts in acidfast stains. CONCLUSIONS: Staining with Uvitex 2B is a suitable method for the diagnosis of I. belli infections. This technique can be used for the diagnosis of three important gastrointestinal parasites (viz, microsporidia, cryptosporidia, and I. belli) responsible for diarrhea in hiv-infected patients.- - - - - - - - - - ranking = 1keywords = diarrhea (Clic here for more details about this article) |
2/4. Recurrent isosporiasis over a decade in an immunocompetent host successfully treated with pyrimethamine.Recurrent isosporiasis in an immunocompetent host is reported. The patient suffered from chronic intermittent diarrhea for over a decade. Multiple short-term administrations of trimethoprim-sulfamethoxazole followed by pyrimethamine, or albendazole combined with tinidazole could not control the relapses. However, treatment with pyrimethamine, 25 mg/d for 20 weeks, was successful.- - - - - - - - - - ranking = 0.5keywords = diarrhea (Clic here for more details about this article) |
3/4. isospora belli infection in a patient with non-Hodgkin's lymphoma.isospora belli infection is frequent in patients with acquired immunodeficiency syndrome in tropical areas. It has also been reported in other immunodepressive diseases, such as lymphoblastic leukemia, adult T-cell leukemia, and Hodgkin's disease. To date, no case of non-Hodgkin's lymphoma-related isosporiasis has been reported in a non-hiv-infected patient. We describe a case of non-Hodgkin's lymphoma with chronic diarrhea due to I. belli. In europe, I. belli can cause severe chronic diarrhea in patients with malignancies whose country of origin is in an endemic area. trimethoprim-sulfamethoxazole can provide rapid and prolonged clinical and parasitologic cure.- - - - - - - - - - ranking = 1keywords = diarrhea (Clic here for more details about this article) |
4/4. Refractory isosporiasis.The authors describe a case of severe debilitating diarrhea due to isosporiasis in a two year old child, a known case of systemic vasculitis receiving prolonged corticosteroids therapy, an association rarely reported previously. It was refractory to treatment with dihydrofolate reductase inhibitor combined with sulfonamide such as cotrimoxazole to which isosporiasis usually responds well and is being described here for clinical interest and uniqueness of its presentation and laboratory findings.- - - - - - - - - - ranking = 0.5keywords = diarrhea (Clic here for more details about this article) |