1/9. Rapid detection and identification of brachyspira aalborgi from rectal biopsies and faeces of a patient.This study reports for the first time the detection of brachyspira aalborgi in faeces and rectal biopsies of a female suffering for 3-4 months of abdominal pain with long-standing mucosal diarrhoea, rectal bleeding and suspected carcinoma of the rectum. After pre-treatment of samples (faeces and biopsies) with a liquid medium (trypticase soy broth-TSB) containing foetal calf serum (FCS, 10%) and spectinomycin and rifampicin (TSB-SR) the first detection of B. aalborgi isolate HBS1 was observed after 48 h in the primary plates of selective blood agar modified medium (BAM) containing spectinomycin and rifampicin (BAM-SR), where growth zones were signalled by a small weakly beta-haemolytic halo. Attempts to subculture spirochaetes in agar media failed. The new HBS1 isolate was only propagated in TSB broth and at electron microscopy it showed 4 endoflagella inserted at each tapered end. The phenotypic characterization of HBS1 demonstrated absence of hippurate hydrolysis, indole production, alpha-galactosidase, alpha- and beta-glucosidase activities in accordance with the B. aalborgi type strain. Rapid identification of B. aalborgi isolate HBS1 was performed directly from faeces and rectal biopsies and subsequently from pure cultures by a genetic method based on 16S dna restriction fragment length polymorphism (RFLP)-polymerase chain reaction (PCR). The sequence of 16S dna amplicon of the isolate HBS1 was found 99.2% corresponding to that of the B. aalborgi type strain. Our results encourage further investigations for the development of a suitable selective agar medium for the isolating and cultivating B. aalborgi from human specimens.- - - - - - - - - - ranking = 1keywords = spirochaete, lyme (Clic here for more details about this article) |
2/9. Intestinal spirochaetosis: a rare histopathological diagnosis and potential light microscopic pitfall.A case of intestinal spirochaetosis is described. Endoscopic specimens of a 52-year-old female revealed a blue basophilic margin of mucosal surface in haematoxylin-eosin stained sections. However, on ultrastructural level, moderate infestation of enterocytic brush border with spirochaetes was found. The pitfalls of histopathological diagnosis of spirochaetosis are discussed.- - - - - - - - - - ranking = 0.99974741382814keywords = spirochaete (Clic here for more details about this article) |
3/9. Symptomatic colonic spirochaetosis in an immunocompetent patient.Spirochaetes are organisms that can infect the colon of people with normal or compromised immune systems. Infected patients can present with a variety of gastrointestinal symptoms, including diarrhoea and rectal bleeding. However, some report a lack of association between specific symptoms and the presence of spirochaetes. It is therefore unclear whether the spirochaetes colonising the colon are true pathogens. diagnosis is typically made by histological examination, with the biopsy specimen showing a band-like growth of spirochaetes adherent to the colonic luminal surface, giving an accentuated brush-border appearance. A course of metronidazole can eliminate the spirochaetes, but treatment might not lead to improvement of symptoms. Owing to the lack of a definite association between symptoms and the presence of spirochaetes, observation without specific antibiotic treatment can be pursued in most patients.- - - - - - - - - - ranking = 4.9987370691407keywords = spirochaete (Clic here for more details about this article) |
4/9. Spirochaetosis of the human rectum associated with an intraepithelial mast cell and IgE plasma cell response.In two patients presenting with mild intestinal symptoms, rectal spirochaetosis was the only morphological abnormality diagnosed by light microscopy. A re-evaluation of the morphological changes using electron microscopy and immunohistochemistry showed certain unusual features: the microorganisms were observed within epithelial cells and in subepithelial macrophages; there were numerous partially degranulated intraepithelial mast cells; and there was a marked increase in the proportion of IgE plasma cells within the lamina propria. Mucosal penetration by the organisms may be responsible for the unusual immune response. In one patient, treatment with antibiotics eliminated the spirochaetes and resulted in a clinical improvement. Spirochaetes should not always be considered as harmless commensals in the colon.- - - - - - - - - - ranking = 0.99974741382814keywords = spirochaete (Clic here for more details about this article) |
5/9. Intestinal spirochaetosis of the colon diagnosed with colono-ileoscopy and multiple biopsies.This paper deals with the clinical history, the histopathological and TEM features of a case of intestinal spirochaetosis associated with ulcerative colitis and tubular adenomas of the colon. It is the fifth described case of intestinal spirochaetosis in sweden, and the first in the literature in which a complete colonoscopy with multiple biopsies has been performed. Intestinal spirochaetosis might lead to minor complaints as distension and vague abdominal discomfort, as in this case, but also to more prominent symptoms such as diarrhoea. Heavy infestation of the gut surface epithelium by spirochaetes was seen in the total colon, but was not found in the distal ileum.- - - - - - - - - - ranking = 0.99974741382814keywords = spirochaete (Clic here for more details about this article) |
6/9. Rectal spirochaetosis.We report four cases of rectal spirochaetosis, one in an active male homosexual. One of the heterosexual patients was referred to the genitourinary clinic by a general surgeon after spirochaetes had been found on histopathological examination of a rectal biopsy specimen. We doubt that most of our cases represent sexual transmission of spirochaetosis, or that the condition causes disease in most people. Rectal spirochaetosis possibly occurs only when the normal flora of the gut are disturbed for other reasons. Most of our patients became asymptomatic after nonspecific treatment, although metronidazole appears to be specific.- - - - - - - - - - ranking = 0.99974741382814keywords = spirochaete (Clic here for more details about this article) |
7/9. Intestinal spirochaetosis: an electron microscopic study of an unusual case.An unusual case of intestinal spirochaetosis is described. The rectum of a 34-year-old male, suffering from Crohn's disease and ankylosing spondylitis, was heavily infested by spirochaetes. Both absorptive and goblet cells were colonized. Spirochaetes were found not only on the luminal surface of these cells, but also within the cytoplasm, in occasional macrophages within the lamina propria and, even more surprisingly, within the occasional Schwann cell. The significance of these findings is discussed.- - - - - - - - - - ranking = 0.99974741382814keywords = spirochaete (Clic here for more details about this article) |
8/9. Colonic tubular adenomas and intestinal spirochaetosis: an incompatible association.Two cases of tubulovillous adenoma and adjacent colonic mucosa were studied in which intestinal spirochaetosis had heavily colonized the non-neoplastic but not the neoplastic epithelium. Ultrastructural and histochemical studies were performed and we postulate that the reason for this disparity is the normal microvillous pattern of the non-neoplastic epithelium which facilitates colonization by the spirochaetes.- - - - - - - - - - ranking = 0.99974741382814keywords = spirochaete (Clic here for more details about this article) |
9/9. The first reported case of intestinal spirochaetosis in japan.A 65-year-old Japanese male consulted Ozuchi Prefectural Hospital (Iwate, japan) on 19 January 1994 complaining of weight loss. Cecal mucosal biopsy material, which was stained with hematoxylin-eosin revealed a thick, basophilic fuzzy fringe covering the surface epithelium. Transmission and scanning electron microscopy observations demonstrated the presence of slightly wavy spirochaetes with tapered ends, which were attached to the surface epithelium of the colonic mucosa via one of these ends. The patient did not display any clinical symptoms of inflammatory bowel disease, and laboratory tests eliminated an immunodeficiency condition. Thus, in the present case, the intestinal spirochaetes appear to be harmless commensals. This paper presents the first reported case of intestinal spirochaetosis in japan.- - - - - - - - - - ranking = 1.9994948276563keywords = spirochaete (Clic here for more details about this article) |