1/16. Haemolytic-uraemic syndrome caused by vero toxin-producing Escherichia coli serotype Rough: K-: H49.The first case of haemolytic-uraemic syndrome (HUS) caused by Vero toxin-producing Escherichia coli (VTEC) which belonged to a novel serotype, Rough: K-: H49, is reported. The case was initially diagnosed as nephropathia epidemica caused by puumala virus, but the subsequent diagnosis of HUS caused by VTEC was made after bacteriological investigation. The strain isolated fermented sorbitol produced VT2 toxin but not enterohaemolysin, nor did it carry the eaeA gene. In VTEC strains, the O antigen, the eaeA gene and enterohaemolysin production have been characterized as virulence-associated factors and believed to have an effect on pathogenesis of these strains to cause haemorrhagic colitis or HUS. The findings of this study demonstrate that there is a need for further studies to evaluate the pathogenetic mechanism of VTEC and need for easy diagnostic methods exploiting other properties than O157 antigen and non-fermentation of sorbitol to find all VTEC in human infections.- - - - - - - - - - ranking = 1keywords = antigen (Clic here for more details about this article) |
2/16. Cerebral malakoplakia associated with Escherichia coli infection.Malakoplakia is an unusual chronic inflammatory disease occurring predominantly in the bladder and only rarely affecting other organs. For the urinary tract, its aetiology has been ascribed to the presence of Escherichia coli, while the very few cases of cerebral malakoplakia which have been reported so far, have mostly occurred in infants in the clinical setting of neonatal herpes virus infection or otherwise in adults in areas of cerebral infarction. We here report a case of E. coli-associated malakoplakia of the brain. It occurred in a 53-year-old man who had undergone long-term corticosteroid therapy and had previously been operated on a cerebral E. coli-associated abscess. This case indicates that malakoplakia of the brain might also be a histiocytic reaction against bacterial antigens of the E. coli family.- - - - - - - - - - ranking = 0.5keywords = antigen (Clic here for more details about this article) |
3/16. Nontoxigenic sorbitol-fermenting escherichia coli o157:H- associated with a family outbreak of diarrhoea.A recent study from germany reported the isolation of E. coli O157:H7/H- from patients with non-bloody diarrhoea and hemolytic uremic syndrome, questioning the role of shiga toxin as the main trait of virulence for human disease. We isolated 6 sorbitol-fermenting E. coli O157:H- strains that do not contain shiga toxin genes. The isolates originated from an outbreak (3 patients, 3 asymptomatic contacts) of non-bloody diarrhoea affecting two families sharing one household. Two children (age 10 months and 2 years) suffered severe diarrhoea over 30 and 10 days, respectively. Their uncle had moderate diarrhoea for 2 weeks. In contrast to the other isolates, the uncle's strain (EH109) did not harbour a chromosomal eae gene encoding gamma-intimin nor the plasmid gene E-hly; it also showed a PFGE pattern that was different from the unique pattern of the other isolates. Employing PFGE, phage typing, and P-gene typing, five of the six stx negative isolates were indistinguishable from the stx 2 positive "Bavarian outbreak strain". The only human serum tested, obtained from one asymptomatic contact, contained antibodies to the O157 lipopolysaccharide antigen. Our finding of five stx negative sorbitol-fermenting E. coli O157:H- isolates (harbouring eae and E-hly) associated with an outbreak of non-bloody diarrhoea supports the hypothesis that Stx production is not obligatory for the pathogenicity of E. coli O157 for humans.- - - - - - - - - - ranking = 0.5keywords = antigen (Clic here for more details about this article) |
4/16. The kinetics of antibody production to antigens of escherichia coli o157 in a pregnant woman with haemolytic uraemic syndrome.Sequential blood samples taken from a pregnant woman with haemolytic uraemic syndrome caused by verocytotoxin (VT)-producing escherichia coli o157 were used to examine the kinetics of serum antibody production to E. coli O157 lipopolysaccharide (LPS), intimin and the conserved region of the translocated intimin receptor (Tir-M). umbilical cord blood and two samples of blood from the newborn baby were also examined for antibodies to these antigens. In the mother, antibodies of the IgM class, specific for E. coli O157 LPS, were produced in the initial stages of the infection, reaching a peak at 9 days after onset of diarrhoea and subsiding 3 days later. High levels of IgG class antibodies, specific for E. coli O157 LPS, were detected 8 days after the onset of diarrhoea and were present at high titres on day 18. serum antibodies of the IgA class to E. coli O157 LPS were not detected. antibodies binding to Tir-M were detected 8 days after the onset of diarrhoea and high titres of these antibodies were still present on day 18. serum antibodies to intimin were not detected in the mother and no antibodies to any of the antigens tested were detected in either the baby's blood or cord blood. This study describes for the first time the kinetics of serum antibody production during pregnancy, to selected antigens expressed by E. coli O157.- - - - - - - - - - ranking = 3.5keywords = antigen (Clic here for more details about this article) |
5/16. Purulent pericarditis in a neonate.A 10-day-old male infant presented with rapid and labored breathing to an outside hospital. An echocardiogram demonstrated a thick, non-free-flowing pericardial effusion. A blood culture drawn grew Escherichia coli. The patient underwent a pericardectomy and pericardial drainage. Samples were taken for acid-fast bacilli, cytomegalovirus antigenemia, fungal, and fluid cultures, all of which were negative. A repeat echocardiogram demonstrated resolution of the pericardial effusion. Antibiotic therapy alone is insufficient to treat purulent pericarditis. The successful treatment of purulent pericarditis requires the combination of pericardial decompression with open drainage and appropriate antibiotic therapy.- - - - - - - - - - ranking = 0.5keywords = antigen (Clic here for more details about this article) |
6/16. Elevated serum prostate-specific antigen due to acute bacterial prostatitis.Elevated serum prostate-specific antigen secondary to acute bacterial prostatitis occurred in 2 patients. This cause of increased PSA has not been documented previously.- - - - - - - - - - ranking = 2.5keywords = antigen (Clic here for more details about this article) |
7/16. meningitis due to two serotypes of Escherichia coli. An infant who recovered.A newborn infant with hyaline membrane disease and aspiration pneumonia developed purulent meningitis on day 19, three days after discontinuation of ampicillin sodium and gentamicin sulfate therapy. Therapy with gentamicin, both systemically and intrathecally, for two weeks was ineffective. During this time each of four specimens of cerebrospinal fluid contained two serotypes of Escherichia coli, namely, O83:H4 and O75:H5. The antibiograms of the two strains were identical, both being susceptible to gentamicin and ampicillin. Treatment with ampicillin resulted in prompt disappearance of the infecting microorganisms and recovery from the infection. One of the strains (O75:H5) produced an antigen cross-reacting with the capsular antigen of haemophilus influenzae type b; the other did not. The patient developed O antibodies in substantial titers against E coli O83 but not against E coli O75.- - - - - - - - - - ranking = 1keywords = antigen (Clic here for more details about this article) |
8/16. Relapsing E. coli K1 antigen meningitis in a newborn.A male infant of 32 weeks' gestational age who presented with recurrent apnoea on the second day of life was shown to have an Escherichia coli K1 antigen meningitis. Relapse occurred 6 days after an adequate systemic course of gentamicin and chloramphenicol and intrathecal gentamicin. This was successfully treated with intraventricular gentamicin and systemic cotrimoxazole. The need to maintain a high index of suspicion for meningitis in the newborn period and to treat adequately the frequently accompanying ventriculitis is emphasized.- - - - - - - - - - ranking = 2.5keywords = antigen (Clic here for more details about this article) |
9/16. The detection of a local prostatic immunologic response to bacterial prostatitis.Although local antibody responses at bronchial, pulmonary and intestinal surfaces have been studied previously a similar response from the prostatic surface has never been described. This investigation demonstrates a distinct local antibody response in the prostatic fluid of 2 patients with bacterial prostatitis. Levels of antigen-specific and total non-specific immunoglobulins A and G were measured at intervals during and following infection for at least 2 years. These studies show that local prostatic immunologic responses are independent of serum responses and specific for the infecting organism. Furthermore, local secretory immunoglobulin a is the predominant immunoglobulin involved in the response to prostatic infection. serum antigen-specific antibody and total serum or prostatic fluid immunoglobulin measurements are in adequate reflections of the prostatic immune response.- - - - - - - - - - ranking = 1keywords = antigen (Clic here for more details about this article) |
10/16. Pigeon and dove eggwhite protect mice against renal infection due to P fimbriated Escherichia coli.Pigeon and dove eggwhite exhibit high level P1 antigenic activity and are potent and specific inhibitors of adherence mediated by P fimbriae of uropathogenic escherichia coli. To evaluate pigeon and dove eggwhite as P fimbrial receptor analogues in the prevention of ascending renal infection, mice were challenged with a P fimbriated E. coli urosepsis isolate suspended in saline alone or in saline plus various inhibitors of adherence, including D-mannose, globoside, and chicken, dove, and pigeon eggwhite. D-mannose inhibited mannose-sensitive adherence but not P fimbrial adherence, and failed to prevent renal infection. Globoside and chicken eggwhite also failed to inhibit P fimbrial adherence; chicken eggwhite had little and globoside had no impact on renal infection. In contrast, dove and pigeon eggwhite eliminated P fimbrial adherence and significantly reduced the incidence and intensity of renal infection. These findings suggest that pigeon and dove eggwhite provide P1-antigen-specific protection against ascending renal infection in mice due to P fimbriated uropathogenic E. coli.- - - - - - - - - - ranking = 1keywords = antigen (Clic here for more details about this article) |
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