1/6. A case of laboratory-acquired murine typhus.We encountered a 32-year-old Korean woman who developed murine typhus in a laboratory. She worked as a technician in a laboratory for rickettsial disease. Immunofluorescence test with rickettsial antigen (R. typhi) was positive at 1: 320 on admission and 1: 1280 after 4 weeks. A dose of 200 mg of doxycycline for 7 days proved to be effective for her condition.- - - - - - - - - - ranking = 1keywords = antigen (Clic here for more details about this article) |
2/6. Comparison of different serotests for specific toxoplasma IgM-antibodies (ISAGA, SPIHA, IFAT) and detection of circulating antigen in two cases of laboratory acquired toxoplasma infection.Two symptomatic toxoplasma infections of laboratory personnel have been serologically followed up for 5.5 and 10 months, respectively. Results obtained by commonly used test systems (indirect fluorescent antibody tests for IgG and IgM antibodies, complement fixation test) were compared with those of two recently developed and improved tests for IgM detection (immunosorbent agglutination assay [ISAGA] and solid-phase indirect haemadsorption assay [SPIHA] as well as with those of a test designed for the detection of circulating antigen (cag-ELISA).- - - - - - - - - - ranking = 5keywords = antigen (Clic here for more details about this article) |
3/6. Laboratory-acquired rocky mountain spotted fever. The hazard of aerosol transmission.Nine patients with laboratory-acquired rocky mountain spotted fever were seen during the period 1971 to 1976. Investigation of each case revealed either definite or probable exposure to an aerosol containing infectious rickettsiae; in no case was there evidence of parenteral exposure either by accidental self-inoculation or by tick bite. These illnesses are believed to represent infection acquired via the respiratory route. This report emphasizes the aerosol hazard of rickettsia rickettsii in the laboratory and discusses the possibility of respiratory transmission of rocky mountain spotted fever in nature. The illness occurred only in personnel who had received either no vaccination or the primary series of the commercial (Lederie) vaccine against this infection. Other personnel who had received the primary series with multiple booster vaccinations demonstrated increased immunity as measured by humoral antibody titers and rickettsial antigen-induced lymphocyte transformation; no cases of clinical disease developed in these multiply-vaccinated personnel.- - - - - - - - - - ranking = 1keywords = antigen (Clic here for more details about this article) |
4/6. Serological studies on a case of laboratory dengue infection.One of the authors (Y.O.), who had previously been immunized with Japanese encephalitis (JE) vaccine, showed symptoms of typical dengue fever 6 days after accidental infection with a newly isolated dengue type 4 virus strain from a patient with dengue hemorrhagic fever (DHF) in thailand. His sera were examined by hemagglutination inhibition (HI), complement fixation (CF) and neutralization (N) tests. The JE N antibody titers of his sera were high even on the first day of the illness and remained almost constant during the next year. antibodies that reacted with dengue viruses were detected from a very early stage of the illness by all three serological tests. In addition, his convalescent phase sera showed high titers against all 4 types of dengue virus. These data suggest that the dengue infection caused secondary stimulation of antigens of flavivirus. Sedimentation analysis of antibodies in Y.O.'s serum (day 9) was carried out and IgM antibody that reacted only with dengue type 4 virus and homologous infecting virus was separated. These findings clearly demonstrated that the laboratory infection of Y.O. was primary dengue infection with dengue type 4 virus.- - - - - - - - - - ranking = 1keywords = antigen (Clic here for more details about this article) |
5/6. epidemiology of an outbreak in a maternity unit of infections with an antigenic variant of Echovirus 11.After an 8-day-old child had died with clinical signs of septicemia, 6 other newborns fell ill. Virus was isolated from various sites from all the 6 children (28 isolations). The agent was identified by cross neutralization tests as an antigenic variant of Echovirus 11. The agent could not be isolated from mothers or nursery staff (49 people). We therefore tried to trace the path of infection by isolating specific IgM and IgG antibodies. A laboratory infection by the agent isolated enabled the time pattern of the serologic immune response to be roughly determined. The data collected indicate that the infection spread through close contact between the affected newborns and nurses working in the newborn room. Rigorous hygienic and isolation measures, initiated immediately, appeared to interrupt the spread of infection.- - - - - - - - - - ranking = 5keywords = antigen (Clic here for more details about this article) |
6/6. Enzyme electrophoresis, sero- and subtyping, and outer membrane protein characterization of two neisseria meningitidis strains involved in laboratory-acquired infections.Two cases of laboratory-acquired infections due to neisseria meningitidis were suspected to have occurred in two French hospitals. The first case occurred shortly, i.e., 3 days, after one strain had been handled by a laboratory technician, and the link between this strain and the strain causing meningitis was easily established. In the second case, infection occurred 3 weeks after 10 strains had been handled by a technician. In this case, it was necessary to use high-resolution markers in order to establish the link between the infecting strain and 1 of the 10 strains handled. The antigenic formulae of the two infecting strains (serogroup:serotype:subtype) were, respectively, C:NT:P1.12 and B:2a:P1.2. Outer membrane protein profile analysis and multilocus enzyme electrophoresis unequivocally confirmed the identity of the respective strains.- - - - - - - - - - ranking = 1keywords = antigen (Clic here for more details about this article) |